Valko test mix C-18
10 mM DMSO solutions of 10 components with logP ranging from -0.5 to 5.5. A chromatogram is supplied using C-18 stationary phase with column dimensions of 3 x 50 mm, and flow rate 1.5 ml/min, applying fast ACN gradient.
£110 + postage
| Gradient tR at pH7.4 | CHI at pH7.4 | Compound |
|---|---|---|
|
1.671 |
18.40 |
Theophylline |
|
1.768 |
23.60 |
Phenyltetrazole |
|
1.911 |
34.30 |
Benzimidazole |
|
2.132 |
42.00 |
Colchicine |
|
2.271 |
51.20 |
Phenyltheophylline |
|
2.475 |
65.10 |
Acetophenone |
|
2.642 |
71.50 |
Indole |
|
2.734 |
77.40 |
Propiophenone |
|
2.932 |
87.50 |
Butyrophenone |
|
3.113 |
96.20 |
Valerophenone |
Valko test mix IAM.PC.DD2
Calibration mixtures can be ordered from REGIS Technologies together with the IAM.PC.DD2 column, or from this site.
£45.99
| Gradient tR at pH7.4 | CHI IAM | Compound |
|---|---|---|
|
5.292 |
49.4 |
Octanophenone |
|
5.064 |
45.7 |
Heptanophenone |
|
4.806 |
41.8 |
Hexanophenone |
|
4.512 |
37.3 |
Valerophenone |
|
4.167 |
32 |
Butyrophenone |
|
3.757
|
25.9 |
Propiophenone |
|
3.156 |
17.2 |
Acetophenone |
|
2.761 |
11.5 |
Acetanilidine |
|
2.184 |
2.9 |
Paracetamol |
HSA and AGP calibration solutions
Use these drug compound solutions in 10mM DMSO to form a calibration plot for HSA and AGP binding. The tables show the percentage bound of each compound and the corresponding log retention time and log K obtained from HPLC experiments.
£329.99
| Compound | HSA % binding | Log retention time | Log K |
|---|---|---|---|
|
Warfarin |
97.90 |
0.40 |
1.50 |
|
Paracetamol |
14.00 |
-0.54 |
-0.79 |
|
Nizatidine |
20.40 |
-0.40 |
-0.60 |
|
Trimethoprim |
37.60 |
-0.13 |
-0.23 |
|
Propranolol |
66.60 |
0.13 |
0.29 |
|
Carbamazepine |
75.00 |
0.13 |
0.46 |
|
Nicardipine |
95.00 |
0.43 |
1.20 |
|
Indomethacin |
99.50 |
0.61 |
1.82 |
|
Diclofenac |
99.80 |
0.59 |
1.92 |
| Compound | AGP % binding | Log retention time | Log K |
|---|---|---|---|
|
Warfarin |
83.20 |
0.34 |
0.67 |
|
Paracetamol |
3.20 |
-0.56 |
-1.49 |
|
Nizatidine |
37.10
|
0.04 |
-0.24 |
|
Trimethoprim |
46.20 |
0.12 |
-0.07 |
|
Propranolol |
86.00 |
0.41 |
0.76 |
|
Carbamazepine |
65.00
|
0.28 |
0.26 |
|
Nicardipine |
87.00 |
0.43 |
0.79 |
|
Indomethacin |
56.00 |
0.19 |
0.09 |
|
Diclofenac |
60.00 |
0.20 |
0.17 |
System suitability test solutions for IAM.PC.DD2 chomatography
The calibration line is used to standardize the gradient retention times on a given column. To check that the IAM.PC.DD2 column represents the true phospholipid binding we need to check the CHI IAM values of a few neutral, acidic and basic drugs. IAM.PC.DD2 columns can last over 5000 injections, but the suitability should be tested after every 200 to 400 injections. The test solutions are also available to order online or send the “Contact us” form to order.
The obtained CHI IAM values for the drug molecules should be within 5 unit of the value listed in the table on the right.
| Compound | Acid/base | CHI IAM |
|---|---|---|
|
Carbamazepine |
Neutral |
27 |
|
Colchicine |
Neutral |
23 |
|
Warfarin |
Acidic |
24 |
|
Indomethacin |
Acidic |
30 |
|
Propranolol |
Basic |
42 |
|
Nicardipine
|
Basic |
45 |
Calibration mixtures
